Preliminary X-ray Diffraction Analysis of Human Chorionic Gonadotropin

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Preliminary X-ray diffraction analysis of human chorionic gonadotropin.

Hexagonal bipyramidal crystals of deglycosylated human chorionic gonadotropin have been grown using the method of vapor diffusion against ammonium sulfate. These crystals grow to nearly 0.4 mm along each axis, diffract to better than 3.5-A resolution and are relatively stable to irradiation. The crystals belong to the hexagonal space group P6(1)22 or enantiomer, and have unit cell parameters a ...

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Crystallization and preliminary X-ray diffraction

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Human Chorionic Gonadotropin

A highly active preparation of human chorionic gonadotropin has been obtained by simple methods of column chromatography on diethylaminoethyl Sephadex and Sephadex G-100. The preparation appeared homogeneous by disc and immunoelectrophoresis, ultracentrifugation, and end group analysis. It assayed at 12,000 i.u. per mg by the rat prostrate gland and accessory sex organ method. The ~20,~ value w...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1989

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)83485-8